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A Robust Portfolio of Resins Offering High Selectivity, Flexibility, and Productivity
Sartorius chromatography resins encompass ion exchange, affinity and mixed mode chemistries coupled with resin beads that offer high flow and superior purification capabilities. Use of our salt-tolerant or mixed-mode resins can lead to significant time and cost savings by eliminating dilution and adjustment steps. Sartorius resins are industry proven to perform packing and purification from benchtop to commercial scale, with several formats to accommodate screening through commercial production needs.
Use of salt-tolerant resins reduce or eliminate the need for dilution or adjustment steps
Unique mixed-mode ligands offer powerful separation capability for the most complex purification challenges
Industry-proven packing and purification performance from process development to commercial scale production
Chromatography Resins for Novel and Challenging Modalities
Sartorius chromatography resins deliver high purity within a simplified process design for small and medium-sized biomolecules. Our resins offer flexible process integration without the need for feedstream manipulation. Our broad portfolio covers our customers’ process requirements and addresses their purification challenges. Our unique selectivity with mixed-mode ligands for both capture and purification will increase the productivity of the existing standard purification platforms.
Ligands | Q, DEAE, CM, STAR AX | Heparin, Lysine, Blue Dye | CMM, HEA, PPA, MEP, HA |
Common applications | mAbs, recombinant proteins, viruses/VLPs, blood fraction, nucleic acids | Recombinant proteins, viruses/VLPs, blood fraction | mAbs, recombinant proteins, fabs, bispecific Abs |
Resin beads | HyperCel, HyperD® | HyperD®, Trisacryl® | HyperCel, Ultrogel® |
Formats | Bulk resin, PRC pre-packed columns | Bulk resin | Bulk resin, pre-packed columns |
Resin | Base Matrix | Ligand | Bead Size | Binding Capacity |
|---|---|---|---|---|
Ceramic polymer composite | Ion exchange | 50 µm | 85 mg/mL* (AEX) | |
Rigid cellulose | Salt-tolerant anion exchange | 80 µm | 100 mg/mL* | |
Rigid cellulose | Mixed-mode | 80 - 100 µm | 40 – 60* mg/mL | |
Rigid cellulose | Mixed-mode | 80 – 100 µm | 20 mg/mL** | |
Rigid cellulose | Mixed-mode | 50 – 80 µm | 50 – 100** mg/mL | |
HA agarose composite | Mixed-mode (hydroxy-apatite) | 60 – 180 µm | 7 mg cytochrome C /ml | |
Trisacryl | Affinity blue dye | 40 – 80 µm | 10 mg/mL* | |
Polymer composite | Affinity heparin | 80 µm | 25 mg ATII/mL | |
Polymer composite | Affinity lysine | 70 µm | - |
* Binding capacity is related to BSA
** Binding capacity is related to IgG
A Range of Resins for Efficient and Cost-Effective Process Design
Ion exchange resins are used in the capture and purification chromatography steps of various biomolecules. Sartorius offers a comprehensive range of chemistries and bead matrices. The resins provide high capacity at short residence times in a flexible range of conductivities up to 12 – 15 mS/cm. Unique salt-tolerant matrices, like HyperCel Star AX anion exchange resin, allow simple process integration without the need for additional feedstream manipulation.
Ligand | Quaternary amine | Primary amine | Diethylaminoethyl | Carboxymethyl |
IEX type | Strong anion exchange | Weak salt-tolerant anion exchange | Weak anion exchange | Weak cation exchange |
Working conductivity | 4-8 mS/cm | Salt tolerant: 2-15 mS/cm | 4-8 mS/cm | Salt tolerant: up to 10-19 mS/cm |
Bead material | Ceramic polymer composite (50 µm) | Rigid cellulose | Ceramic polymer composite (50 µm) | Ceramic polymer composite (50 µm) |
DBC at 10% breakthrough | BSA ≥85 mg/mL | BSA ≥100 mg/mL | BSA ≥85 mg/mL | IgG ≥60 mg/mL |
Q Ceramic HyperD®
Q Ceramic HyperD® is a strong anion exchanger that is suited for the removal of anionic impurities (i.e., host cell protein and DNA). Its 50 µm base bead offers a high resolution for purification applications when required.
DEAE Ceramic HyperD®
DEAE Ceramic HyperD® is a weak anion exchanger for purification. Changes in conductivity and pH can optimize purification steps by utilizing the charge change on protein and ligand.
CM Ceramic HyperD®
CM Ceramic HyperD® is a weak cation exchanger for polishing applications. Changes in conductivity and pH can optimize purification steps by utilizing the charge change on protein and ligand.
HyperCel STAR AX
HyperCel Star AX is a salt-tolerant anion exchanger with a primary amine as functional group. It can easily be integrated into a purification workflow without feedstream manipulation.
Find the Right Solution for Your Affinity Chromatography Process
Sartorius offers multiple affinity chromatography resins with high binding capacities in a variety of selectivities to purify recombinant proteins, albumin, enzymes coagulation factors (ATIII), plasminogen and growth hormones.
Applications | Coagulation factors (ATIII), growth hormones and enzyme purification | Plasminogen, recombinant protein and glycoprotein purification | Albumin, enzymes, recombinant proteins and purification |
Dynamic binding capacity (10 % BT) | > 25 mg/mL | - | > 10 mg/ml |
미립자의 평균 입도 | 80 µm | 70 µm | 60 µm |
Working pH range | 3 –13 | 3 –13 | 1 – 10 |
Operating pressure | <3 barg (44 psig) | <3 barg (44 psig) | - |
Heparin HyperD®
Heparin HyperD® M resin is suited for the purification of heparin-binding proteins like coagulation factors, growth hormones, lipoproteins, and DNA/RNA processing enzymes.
Lysine HyperD®
Lysine HyperD® affinity resin is used for the purification of lysine-binding proteins such as plasminogen. It provides high protein-binding capacities at high flow rates.
Blue Trisacryl® M
Blue Trisacryl® M is an affinity resin used for the purification of enzymes, interferons, and some coagulation factors as well as for the removal of albumin. The resin uses Trisacryl® based beads which carry the blue dye ligand.
New Purification Challenges Require a Different Approach
Novel biopharmaceutical targets such as nanobodies, plural-specific mAbs, and fusion proteins require unique selectivities to achieve high product purity. Mixed-mode resins leverage multi-modal separation mechanisms such as IEX and HIC to achieve higher selectivity and purity in a single step than in traditional single-mode chromatography.
Sartorius offers a broad range of selectivities to address challenges with a high concentration of contaminants with the intensified feedstreams, aggregates issues with newer modalities, and to remove challenging process or product-related impurities. Achieve reduced process costs by streamlining pre- or post-diafiltration steps.
Modes | CEX HIC | Hydrophobic charge | AEX HIC | AEX HIC | CEX Pseudo-affinity |
Application | B/E antibody | Capture of antibodies in physiological conditions (pseudo affinity) only for MEP HyperCel |
| ||
Dynamic binding capacity | IgG | Human IgG | BSA | BSA | Cytochrome C |
Average particle size (µm) | 50-80 | 80-100 | 80-100 | 80-100 | 60-180 |
* 10% BT
** 50% BT
CMM HyperCel
CMM HyperCel is a hydrophobic cation exchanger recommended for the capture of antibodies, antibody fragments, and recombinant proteins. It allows the separation of proteins with similar pI by modulation of pH and conductivity.
MEP HyperCel
MEP HyperCel provides unique selectivity for monoclonal antibody capture or purification to remove aggregates, HCP, and DNA. The ligand facilitates the binding of target proteins at neutral pH under moderate salt concentrations.
HEA HyperCel
HEA HyperCel is a mixed-mode ligand with a less hydrophobic hexylamine chain allowing protein binding at a moderate salt concentration under neutral pH.
PPA HyperCel
PPA HyperCel exhibits a stronger hydrophobicity with an aromatic ligand, allowing protein binding at a moderate salt concentration under neutral pH.
HA Ultrogel®
HA Ultrogel® hydroxyapatite is a composite material of cross-linked agarose and microcrystalline hydroxyapatite enclosed in the agarose matrix. The material shows mixed-mode functionality based on cation exchange and metal affinity in the hydroxyapatite structure. It is ideally suitable for general impurity removal.
