Experience the Difference of Label-Free Octet® BLI Systems
For over 20 years, we have dependably supported our customers by creating established fluidic-free Biolayer Interferometry (BLI) platforms, consumables, and software. These products are trusted by scientists worldwide to support their bioanalytical needs for protein interaction analysis spanning from drug discovery to antibody engineering to bioprocessing and quality control (QC).
Our dedication to quality is reflected in an install base of more than 3,500 customers and is well-documented in more than 7,000 customer publications. Over the years, our top priority has been providing exceptional technical and scientific support, ensuring you can quickly and effectively utilize our products to achieve your experimental goals.
We continue to shape the future with Octet®, the only brand currently combining both industry-leading label-free technologies – Biolayer Interferometry (BLI) and Surface Plasmon Resonance (SPR) for real-time label-free measurements of binding kinetics and affinity or to determine the active concentration of an analyte.
Performance, flexibility and confidence for real-time label-free, high-quality binding kinetics and analyte quantitation.
Octet®️ BLI systems offer an advanced, fast, robust, and fluidics-free approach for protein-protein and protein-small molecule analysis. The Octet®️ BLI platform allows direct detection of specific proteins or drug molecules - even in complex mixtures and unpurified samples such as cell culture supernatants and lysates.
Always opt for the genuine Octet® platform to empower your research by enabling faster time to market, quicker project completion and greater project capacity.
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Grows with your biomolecule characterization needs
The perfect balance between cost and throughput with given leeway
Unmatched flexibility and versatility in protein analysis
Monitors up to 96 biosensors simultaneously, enabling label-free detection for protein quantitation and kinetic characterization at unmatched speed
Ideal for high-throughput applications that demand high sensitivity and low sample volume requirements
Affordably priced label-free analysis for individual researchers on any budget
Available in a wide range of surface chemistries for use in a diverse set of biomolecular applications.
A wide range of compliance tools for developing validated assay methods on the Octet® BLI platform.
Facilitate data acquisition and data analysis in laboratories working under GMP, GLP and 21 CFR Part 11 regulations.
Receive outstanding assistance for both technical issues and assay-related enquiries.
Global - Get up to $190K (€160K) off when you bundle Octet® BLI and SPR systems
Proven countless times – the genuine Octet® BLI is an industry-leading technology with more than 7000 peer reviewed publications covering a large variety of research areas and applications. The Octet® Publication Database provides an easy way to search scientific publications that reference and/or cite the use of our Octet® products for label-free biomolecular interaction analysis in real time.
Literature references covering a large variety of research areas and applications
Thousands of researchers worldwide use Octet® systems in diverse fields ranging from drug discovery to biopharmaceutical development and academic research to determine binding specificity, active analyte concentration, affinity, or binding kinetics in real-time.
Discover what users of genuine Octet® BLI have to say about this indispensable tool that has helped them advance their research and accelerate the development of new therapeutics and diagnostics.
Application Area: High-throughput antibody characterization and small molecule analysis
"The Octet® RH96 lets our team carry out quantitation at high-resolution that is comparable to an HPLC and rapid throughput. It allows shortening of our experimental timelines and less cost for cell line development projects. Our team uses it for label-free, real-time data generation and it's a facile instrument to use that provides superior quality data at a very cost-efficient price."
- Sankalp Gupta, Sanofi
Application Area: High throughput mAb characterization and small molecule analysis
"We use it for label-free, real-time data generation. The Octet® RH96 is a facile instrument to use and provides superior quality data at a very cost-efficient price."
– Kesh Prakash, Emergent BioSolutions
Application Area: HTS drug development screening tool
"The Octet® RH96 allows quantitation with high resolution comparable to an HPLC and rapid throughput. The platform allows shortening of our timelines and less cost for cell line development. The instrument is very robust, easy to use and is well supported by an experienced support staff of application scientists, software engineers, service engineers and sales. Integration of the instrument into an automation system was completely supported by the vendor."
– Glenn Yamasaki, Bayer Healthcare
Application Area: IgG Quantitation in Cell Line Development
"Easy to use instrument [Octet® R8], good results. Easy to explain to new users. Great support."
– Julius Grzeschik, Ferring Pharmaceuticals
Application Area: Drug discovery
"Octet® is very easy to use system for fast screening of protein - small molecule interaction studies. Easy to maintain and definitely value for the money because of time saved in measurements time."
– Juha Määttä, Tampere
"State of the art technology"
– Hailan Yao, Roskamp Institute
The Octet® R Series offers an advanced, fluidics-free approach to bimolecular analysis, with a wide variety of off-the-shelf biosensors for rapid binding kinetics and quantitation analysis. These systems utilize Sartorius’ genuine label-free Biolayer Interferometry (BLI) technology, enabling direct detection of specific proteins and other biomolecules — even in complex mixtures like crude cell culture supernatants and lysates.
The Octet® BLI systems monitor binding events in real time to calculate on rates (ka), off rates (kd) and affinity constants (KD). The superior sensitivity enables measurement of low molecular weight molecules down to 150 Da. The following example shows the kinetic interaction analysis of a small molecule.
Figure 1. The kinetic analysis of between a ligand, biotinylated-carbonic-anhydrase, and the small molecule analyte with Super Streptavidin (SSA) Biosensors at 1000 rpm and 25°C on an Octet® R8 system. Working solutions were prepared in serial dilutions of 0.1, 0.3, 1.0. 3.0, 10.0 µM (n=4), respectively. Data was processed and curve fitted using a 1:1 binding model.
Slow off-rate measurements show minimal changes in signals in the dissociation step requiring a combination of stable baselines and low baseline drifts for accurate measurement of the dissociation phase.
In addition, capturing measurable dissociation of the interaction may take hours of (> 2 hr data acquisition) specifically at the dissociation phase of higher affinity interactions that exhibit very slow off rates. Therefore, measuring longer dissociation times is also critical for measuring high-affinity interactions.
The Octet® R8 system simultaneously enables measurements of up to 8 samples in a single unattended run. Combined with a unique microplate cover that keeps samples intact by minimizing evaporation, it allows scientists to collect accurate measurements with evaporation control for up to 12 hours.
The measurement of one high-affinity interaction measured in triplicates in a long dissociation experiment on the Octet® R8 system is demonstrated below. Full kinetic characterization of an antibody-antigen interaction was run three times with a total run time of 11 hours.
Figures 2 and 3 provide two examples of long dissociation measurement over several hours to allow the detection of very slow off-rates.
Figure 2. A high-affinity interaction between an IgG and Fab measured in triplicates that required a long dissociation step time of more than three hours was measured using an Octet® R8 system. The total experiment run time was 11 hours for measurements made in triplicate.
Figure 3. Kinetics of high-affinity interaction between Human Epidermal Growth Factor (HER2) protein and anti-HER2 monoclonal antibody analyzed in the Octet® R8 system demonstrating reliable high-resolution kinetic data.
When investigating biological mechanisms and developing new therapeutics, BLI and SPR offer unique advantages
A comprehensive guide for designing and performing assays that accurately measure the binding kinetics of biomolecular interactions and analyte concen...
Current regulatory guidelines for assay validation were harmonized for kinetic-based assay to derive a common set of parameters.
From antibody quantitation, ligand binding characterization to potency assays, Sartorius offers a wide range of GxP compliance tools for developing validated assay methods on the genuine Octet® BLI platform.
The United States Pharmacopeia (USP) has recently released a new general chapter <1108> which for the first time cites the use of biolayer interferometry in key ligand binding applications that provide more precise kinetic data and that can measure a relatively wide range of binding affinities. Read More
This service enables the Octet® BLI users to sample multiple lots of a biosensor during assay qualification and validation and reserve a well-characterized lot for purchase. Hence, customers can order up to five different biosensor lots, up to 20 trays from each lot for evaluation, and reserve up to 40 additional trays for future purchase.
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Octet®️ BLI assays can help identify potential safety concerns and improve the accuracy of drug evaluations. Here are several examples of companies, including Pfizer, AstraZeneca, or Roche, who have utilized such assays to generate data submitted as part of the supporting information for the drug’s approval with regulatory bodies such as the FDA or the EMA.
Explore Examples of Approved Drugs Using Octet® BLI
Biolayer Interferometry (BLI) is a label-free technology for measuring biomolecular interactions. It is an optical analytical technique that has gained popularity in the fields of biochemistry, biology, and medical research due to its real-time, high-throughput analysis capabilities.
Biolayer Interferometry (BLI) is a label-free analytical technique commonly used to study biomolecule interactions. It is based on the principles of optical interferometry, where changes in the thickness of a biological layer constructed on a biosensor tip cause shifts in the interference pattern of white light reflected from the biolayer. This shift in the interference pattern correlates to real-time layer thickness changes, which allows for monitoring molecular binding and dissociation.
BLI is especially useful in providing quantitative data on the analyte concentration, binding kinetics, binding affinity, and specificity of biomolecular interactions without requiring fluorescent or radioactive labeling. This makes it a popular choice in various fields, such as drug development, antibody testing, and protein analysis.
In BLI, a biosensor tip is dipped into a solution containing biomolecules. These molecules bind to the biosensor, forming a thin biological layer. The interference pattern changes as more molecules bind to the biosensor, causing a shift in the wavelength of the reflected light. By monitoring this shift, scientists can track the binding process in real-time. This allows them to measure the rate of association (ka) and dissociation (kd), as well as the binding affinity (KD) of the interaction, providing valuable insights into the biomolecular interaction of a wide variety of molecules.
Biolayer Interferometry (BLI) offers several advantages over other biomolecular binding analysis methods such as Surface Plasmon Resonance (SPR) and Enzyme-Linked Immunosorbent Assay (ELISA). Here are some of the key benefits:
Biolayer Interferometry (BLI) has great potential in various fields, including biomedical research, drug discovery, and diagnostic development. It is a highly effective tool for antibody characterization and for exploring interactions between proteins and small molecules, which can significantly aid in therapeutic development.
BLI can be useful in biosimilar production, assessing vaccine efficacy, and quality control for protein therapeutics. It is also beneficial in studying cellular signaling and gene regulation through interactions between ligands and receptors, and nucleic acids and proteins. In the biopharmaceutical industry, BLI can play a critical role in ensuring quality control and can help in the development of diagnostic tools for detecting disease biomarkers, highlighting its wide-ranging applications in life sciences.
BLI biosensors operate on the principle of interferometry employing a white light source, which emits light across a broad range of wavelengths. The interference between the light reflected from the top of the biological surface on the sensor tip and the light reflected from an internal reference layer creates an interference pattern. This pattern is captured by a detector, typically a spectrometer or photodetector, and is used to measure changes in the interference signal. These changes in the interference pattern are directly related to the biomolecular interactions occurring on the sensor surface.
Biolayer Interferometry is a powerful and versatile technology that provides a valuable tool for studying biomolecular interactions. Its ability to provide real-time, label-free analysis makes it an invaluable tool in advancing the understanding in biochemistry, biology, and medical research, contributing significantly to the development of new drugs and therapies.
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