Chinese hamster ovary (CHO) cells are vital for therapeutic protein production in biopharmaceutical research and development and are consistently in high demand. Sartorius’ portfolio of CHO Cell Culture Media and Feeds includes a variety of high-performance, off-the-shelf culture media and feeds for CHO cell lines. The media and feeds are ideal for protein production using CHO-DG44, CHO-K1, CHO-S and CHO-GS cell lines and are suitable for research and routine large-scale GMP manufacturing.
The portfolio offers high performance, scalability, and high quality.
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4Cell® SmartCHO media and feeds are formulated to provide robust performances in batch/fed-batch processes in various CHO clones (DG44, K1, S, and GS).
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TCX10D medium supports cell growth and production of recombinant proteins and antibodies in suspension culture.
4Cell® SmartCHOpe media and feeds are designed for perfusion processes and support intensified process formats.
We were looking for chemically defined media to use with our CHO-K1 cells in suspension. After testing several Sartorius media, we selected 4Cell® SmartCHO for protein production. It has proven to be the best media used at Adipogen Lifesciences to date: it allowed cells to grow at a higher density compared to our current medium, resulting in a fourfold increase in protein yield. This makes it very useful for our stable clone production.
Learn how screening media and feeds with the Ambr® systems can optimize your process to achieve titers of 5 g/L.
Basic Feed improves recombinant protein production in suspension culture by increasing cell density compared to batch processes.
TCX7D Feed is ideal for fed-batch processes, and, in combination with TCX10D, provides optimal nutrition for CHO-GS cells.
Ideal additives to 4Cell® SmartCHO Production Medium. 4Cell® SmartCHO Feed Medium A (FMA) and Feed Medium B (FMB) boost fed-batch productivity 3- to 5-fold compared to batch culture conditions.
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4Cell® SmartCHOpe Feed Medium A (FMA) and 4Cell® SmartCHO Feed Medium B (FMB) are the ideal additives to 4Cell® SmartCHOpe Production Medium.
PowerCHO™ 2, PowerCHO™ GS and PowerCHO™ Advance are chemically defined, serum-free media with animal component-free. They are designed to support growth of CHO K1, DXB11 and DG44. For therapeutic bioprocessing applications, these protein-free media formulations facilitate both downstream purification and regulatory compliance.
Free of glutamine, hypoxanthine, thymidine and phenol red.
ProCHO™ 4 and 5 are serum-free, protein-free and animal component-free media for supporting high-density CHO cells in suspension.
4Cell® XtraCHO Media System was developed for our first generation of cell line development services. It is available for legacy applications needing continuity of supply.
CHO cells are typically cultured in Ham's F12K or DMEM supplemented with 10% FBS. However, in commercially manufacturing, chemically defined and animal-derived component-free media are recommended for culturing Chinese hamster ovary (CHO) cell lines for consistency and quality assurance of the end product.
CHO cells became popular to study genetics because they have a low chromosome number. As a result, CHO cells have been used for a long time and we know a lot about their genetics and growth characteristics.
Serum-free media are media designed to grow a specific cell type or perform a specific application in the absence of serum. Serum-free media are an important tool allowing scientists to culture cells with a defined set of conditions while minimizing variables.
Some advantages of using serum-free media include easier purification and downstream processing, precise evaluations of cellular function, increased definition, more consistent performance, and a more consistent supply chain.
The CHO Platform service package brings together CLD, cell banking and testing services for protein expression.
Sartorius offers a complete scalable bioreactor platform from cell and strain line development to commercial manufacturing.
Enable real time monitoring for cell cultures while reducing the risk of contamination
High-quality solutions designed and validated for the cryopreservation and cold storage of cells, including stem cells, T cells, and PBMCs.
Sartorius’ media services team can provide cell culture solutions specific to your unique processing needs.
In this eBook, our experts reveal valuable insights to help you develop an optimal cell culture media strategy for your bioprocess.
In this webinar, Sartorius and Sonnet BioTherapeutics discuss the benefits of the Sartorius CHO Platform of integrated products and services.
Fast Development of Coronavirus Vaccines
Learn about a methodology that combines data-rich experiments with advanced data analytics for High-Throughput Media Optimization for CHO Cell Lines.
Get consistent performance with easier purification andprocessing – and fulfill regulatory requirements.
Learn more about the origin of CHO cell lines and how testing different cell culture media can help optimize your upstream process.
Learn more about an intermediate harvesting process that enables higher productivity and longer cultivation time compared to a standard fed batch in 4...
Browse CHO media, feeds, and services that meet your needs. We provide what’s vital to your cells — and your success.
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