Speed Up Your Entire Workflow With iQue® High-Throughput Screening (HTS) by Cytometry
Traditional flow cytometers can analyze a limited number of samples but fall short for rapid screens of antibodies or chimeric antigen receptor (CAR) T cells.
The iQue® 3 HTS Cytometer addresses this critical gap as a data generation powerhouse for high-throughput screening applications. Its unique advantages lie in its speed and user-friendliness, from setup to acquisition and analysis, dramatically boosting productivity. Your results will be ready by the time you finish a quick coffee break.
Featuring a patented sampling method, the iQue® 3 enables the fastest sample acquisition in the industry. It supports 96-, 384-, or 1536-well plates and facilitates continuous plate loading through integration with popular robotics systems. Coupled with integrated analysis and novel visualization tools, this platform delivers quality results faster, so you can develop therapeutics sooner.
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Robotic integration capabilities enable high throughput applications.
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Onboard vortexing just prior to sampling ensures proper resuspension and delivers improved QC results and consistency.
Minimal volume sampling capabilities, as little as 1 μL, conserve samples and reagent costs.
Samples are delivered to detectors in an air-gap delimited stream as part of a patented, rapid microvolume sampling process.
Extended run time on cartridges allows for unattended multiple plate processing. Liquid level detection and user-definable low-level warnings help ensure there are always enough reagents to complete planned runs.
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Simultaneous Cell Phenotyping and Secreted Protein Detection
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Relieve capacity bottlenecks throughout your entire experiment with the fastest plate sampling, integrated analysis, and novel data reduction tools
Conserve precious samples and reagents by sampling as little as a single microliter from miniaturized assay volumes
Generate more biologically relevant data through simultaneous measurement of phenotypes and cytokines in the same well
Enjoy a simple, multi-user environment with walkaway automation features including 48 hour uninterrupted run times and automated QC bead vortexing on-...
Dramatically reduce time to decision with novel visualizations that facilitate the fastest path to actionable results, even from complex data sets
See the iQue® 3 in action and discover how it can transform your research
To efficiently characterize disease states and to develop a clear picture of how single cells respond to a stimulus, whether it’s a small molecule, antibody, cytokine, or other entity, quantitative measurement of multiple biological readouts is required.
iQue® HTS by Cytometry Applications enable the assessment of multiple cell types and a range of cellular parameters in a single well, allowing for a streamlined high-throughput workflow, improved data coherence and a highly informed data set.
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Increase data throughput and quality by multiplexing antibody binding, function, and titer across the process.
Assess multiple cell parameters faster, with fewer cells and less reagents.
Streamlined combined workflows and protocols that are simple and easy to follow providing greater biological insights
Understand the Complexities of the Immune System with the iQue® HTS Platform
Analyze cell health, viability and proliferation on a cell-by-cell basis with the iQue® HTS Platform
iQue® Forecyt® Software —Rapid Data Visualization and Interpretation
The integrated iQue® Forecyt® software addresses the analysis bottlenecks associated with multi-parametric data generation by enabling rapid data visualization and interpretation.
Reduce data analysis times from days to minutes by having all of your data analyzed as soon as the plate is done sampling.
Optimized for the iQue® HTS Platform
iQue® Kits, most of which have been developed to work with no-wash protocols, are packaged to suit a wide range of throughput needs.
These kits, in conjunction with an iQue® HTS Cytometer and iQue Forecyt® Software, provide an integrated screening solution enabling high content, high throughput screening of cells and beads for deeper insight into complex biologies.
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The iQue® 3 HTS by Cytometry Platform is available in 3 configurations to address a wide range of screening and experimental applications. The entry level 2 laser iQue® 3 BR is equipped with a blue and a red laser ideal for applications which do not require many parameters, but, can take advantage of the speed the iQue® HTS Platform is famous for.
Two additional 3 laser configurations increase the flexibility and accommodate larger multiplexing needs depending on the specific fluorphores. The iQue® 3 VBR includes Violet, Blue, and Red lasers, while, the iQue® 3 VYB replaces the red laser with a yellow laser.
iQue® 3 Blue-Red (BR)
iQue® 3 Violet-Blue-Red (VBR)
iQue® 3 Violet-Yellow-Blue (VYB)
Catalog No.
91375, 91381 (HD)
91377
91379
Lasers
488nm, 640nm
405nm, 488nm, 640nm
405nm, 561nm, 488nm
Detection Channels
8
15
Plate Types
96- and 384-well, 384- and1536-well (Blue-Red HD)
96- and 384-well
Min Sampling Time
< 5 minutes/96 wells, < 20 minutes/384 wells
Min Sample Aspiration
1 µL
Detection Rate
Up to 35,000 events/second
Dynamic Range
> 7 Decades
Optional Products
Cat. No.
Forecyt® Enterprise Edition with 5 Floating Client Licenses
91131
Robotic Integration API License
90208
Qmax Refill Module
91309
In-solution, cell-based assays are the way forward and the ability to do so with the iQue screener in a 384-well high-throughput format has been extremely useful to us. We can now use one third of the materials and screen 4 times more antibody clones in the same amount of time, obtaining more compelling data for ourselves and our clients.
The iQue Screener allows us to run high throughput screening assays against primary cell types monitoring drug action in a way that simply wasn't possible.
In short, the iQue offers flow cytometry capabilities at real high throughput, making it the perfect instrument for cell and bead based screenings.
Stay current, ensure productivity, manage costs and avoid downtime
Keeping your instrument covered under a Sartorius service contract is an important part of ensuring productivity, managing cost and avoiding downtime. A Sartorius service contract has these key benefits:
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A Guide to HTS Cytometry Assays and Workflows
With over 400 references worldwide, the iQue®️ HTS Platform helps researchers explore new areas of study and address questions fundamental to life science research.
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Use the dynamic, interactive fluorescence spectraviewer tool to see compatibility with over 1,000 fluorophores on the different iQue® 3 configurations.
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Get answers to the most common questions about Sartorius HTS cytometry instruments including critical information regarding screening measuring, speed, plate handling, compliance, and more.
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Cell or particle size and complexity are the non-fluorescent measurements along with fluorescent detection which can be used to identify and quantitate specific proteins both on the surface of cells as well as detection of secreted proteins or signaling molecules.
The iQue® flow cytometer uses a proprietary Air-Gap sampling method which can sample an entire 96 well plate in 5 minutes, or a 384 well plate in 15 minutes. But, the speed doesn’t stop there. The included Forecyt® software makes analysis a breeze by utilizing dynamic plate level data analysis and visualization making quick work of identifying the samples of interest.
Yes. It is compatible with all the major automation vendors systems. An iQue® 3 automation client software license is available allowing the plate handling robot to control the iQue® 3 to load plates, begin a run, and remove plates when the run is completed.
The enhanced rinse station is a smart fluidics system that constantly measures the volume of each buffer or solution to avoid failed runs due to insufficient reagents. It utilizes special cartridges for each fluid which minimize evaporation and can be sealed and placed in the refrigerator when not in use to extend the on-board lifetime when not in use.
Yes. There is a software module available which provides support for labs which require an additional level of traceability for experiments run on the iQue® platform. Among other features, it includes customizable user roles to help tailor the level of documentation and access to information based on specific needs or functions.
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