Application Note: Second Generation Anti-Human IgG Fc Capture Biosensors for Affinity Characterization of Human IgG-Antigen Interactions and Quantitation of Human IgGs or Human Fc Region Containing Proteins
Octet® AHC2 biosensors, second generation, are designed to expand usage of anti-human Fc capture biosensors into a diverse range of applications involving human IgG as the ligand. Developed to enhance human IgG ligand loading response, they enable both human IgG titer determination and antigen binding kinetics characterization.
This informative application note explains how the biosensors were found to exhibit 2.0X enhanced ligand capture response with higher antigen binding response observed in all kinetics case studies. The higher antigen response implies higher sensitivity and a potential expansion of Octet® applications that utilize anti-human capture biosensors to small antigens such as small proteins and peptides. In addition, the enhanced ligand capture resulted in an expanded dynamic range of up to five-fold over first-generation biosensors when performing human IgG quantitation assays. Moreover, these new biosensors can be re-used multiple times, allowing for significant cost savings.
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