Octet^® Bio-Layer Interferometry as a Tool for Determining Nanoparticle Vaccine Construct Design, Stability and Antigenic Efficiency

Nanoparticle (NP) vaccine and antigen delivery platforms have emerged as a promising approach due to their ability to interact with immune components and induce humoral and cellular immune responses.

NP arrangements have also been shown to increase antigen stability and to provide greater flexibility allowing the incorporation of diverse antigen conformations and immunostimulants compared to traditional vaccines. However, NP formulations’ optimal performance depends on antigen presentations and nanoparticle physicochemical properties such as core size, charge, surface chemistry, and composition.

In this article, we review a few selected examples to showcase how Octet^® Bio-layer Interferometry platforms can be used to measure NP attributes such as antigen spacing, presentation, and stability to select ideal NP vaccine candidates. Additionally, we discuss how quantitation capabilities on the Octet^® system are used to determine downstream vaccine efficacy by measuring NP vaccine-derived humoral responses.