Lab-Scale Harvest and Clarification of Lentiviral Vectors Using Sartoclear Dynamics^® Lab Filtration Systems

In this study, the use of Sartoclear Dynamics^®️ Lab V50 (Sartorius) comprising the diatomaceous earth (DE) filter aid was evaluated for clarification of lentiviral vectors (LV) produced by HEK293 suspension cells. The effect of the DE concentration and incubation time on filtration time, yield, and turbidity reduction was analyzed in a design of experiment (DOE) approach.

Moreover, the use of Sartoclear Dynamics^®️ Lab V50 was compared against the standard laboratory method (centrifugation and subsequent filtration) to examine if DE can effectively clarify LV as this filter aid is easy to handle.