Lab-Scale Harvest and Clarification of Lentiviral Vectors Using Vacuum Filtration Units

In this study, Sartolab^®️ RF50 and Sartoclear Dynamics^®️ Lab V50 (Sartorius), as well as other manufacturers’ filters, named products A, B, and C, were evaluated for clarification of lentiviral vectors produced by transient transfection of HEK293T/17 SF suspension cells.

Filtration performance was evaluated regarding the volume filtered, filtration time, filter capacity, flux, turbidity reduction, and removal of contaminants, as well as lentiviral vector titer recovery.