Application Note: Lab-Scale Clarification of Mammalian Suspension Cultures Using Sartoclear Dynamics^® Lab V Kits

This study evaluates a novel method for lab-scale clarification of mammalian cell culture supernatants. The filtration step is conducted prior to purification of monoclonal antibodies with protein A affinity chromatography. In a conventional clarification process, cells are removed by a time-consuming centrifugation step followed by filtration. We show that the Sartoclear Dynamics^® Lab V Kit is not only a significant time-saving method compared with the conventional clarification process. It also yields an improved clearance rate that we observed in combination with high flow rate filtration. For clarification of large-volume cell cultures, Sartoclear Dynamics^® Lab V Kits are an attractive addition to standard lab instrumentation to increase productivity and throughput prior to protein purification.

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