High-Throughput Cell Count & Viability Using the iQue® Platform

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  • Viable, High-Throughput Cell Density Determination Using the iQue® Advanced Flow Cytometer

Application Note: High-Throughput Cell Count & Viability Using the iQue® Platform

 Authors: Nicola Bevan, Andrea Gomez-Donart, Rathangadhara Nammalwar, and Mark Carter | Last updated: January 2023

Overview

Traditional methods for measuring cell count and viability are often low-throughput, time-consuming and lacking in linearity. Advanced flow cytometry provides a fast, accurate and reproducible solution for cell viability and density quantification, which is essential for efficient biologics development processes. 

This application note documents use of the iQue® Cell Count and Viability Kit in combination with the iQue® Advanced Flow Cytometer to accurately quantify cell count and viability in 96- or 384-well plates. Presented data shows how the iQue® Cell Count and Viability Kit - alongside the integrated iQue Forecyt® analysis software - can be effectively used with various non-adherent cell types for rapid and accurate quantification, requiring small cell volumes. Data also demonstrates how this kit can be easily, rapidly and successfully utilized for a cell line development workflow.

  • Document type: Application Note
  • Page count: 8
  • Read time: 11 minutes

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Key Takeaways:

  • How to achieve reproducible, quantitative analysis of cell count and viability
  • Reducing sample preparation time with a simplified workflow

This Resource is Designed for:

  • Cell line development engineers
  • Drug developers
  • Analytical scientists
  • R&D scientists

Applications Supported:

  • Cell line development
  • Biotherapeutic discovery and development
  • Antibody discovery and development

iQue® Cell Count and Viability Kit Workflow

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