Application Note: A High-Throughput Radioactivity Free Cell Mediated Cytotoxicity Assay

Current assays that measure cell-mediated cytotoxicity (CMC), such as the chromium release assay, are difficult to perform on large numbers of samples, can only report on a single biological readout like cell membrane integrity, and cannot differentiate between effector and target cells. Although flow cytometry assays, can assess CMC at the level of an individual cell and enable discrimination between effector cells and target cells they are time consuming, making them unsuitable for a high throughput screening (HTS) assay.

The Intellicyt^® iQue Screener PLUS platform enables rapid radioactivity-free CMC assays which can differentiate target cells from effector cells and provide additional readouts for a deeper understanding of target-effector interaction in a single miniaturized assay format.